What makes serum lipemia

Differences exceeded the LCSI for protein, albumin, and calcium in the comparison of high-speed centrifugation and LipoClear. A large number of biochemical parameters were tested, and the methods most commonly used for lipemia removal in laboratories were compared. We found no statistically significant differences between ultracentrifugation and high-speed centrifugation for potassium ion, protein, albumin, creatinine, urea, alkaline phosphatase, calcium, gamma-glutamyl transferase, and aspartate aminotransferase.

Sodium ion, chloride ion, glucose, bilirubin, and alanine-aminotransferase showed statistically significant differences, but none of these exceeded the LCSI. Analysis of the lowest fraction at the bottom would be suitable for measuring the concentrations of the biochemical parameters included in this study.

High-speed centrifugation is easy to perform in the laboratory, even in the emergency laboratory, where lipemic samples often arrive. In accordance with our findings, Dimeski and Jones [ 9 ] reported no significant differences between ultracentrifugation and high-speed centrifugation, using metrological criteria based on the coefficient of variation of data for sodium ion, creatinine, urate, protein, lactate dehydrogenase, and magnesium.

We found differences in protein, calcium, and aspartate aminotransferase in the comparison of high-speed centrifugationa and 1,1,2-trichlorotrifluoroethane, and in protein, albumin, and calcium in the comparison of high-speed centrifugation and LipoClear. This may be because of incomplete removal of the interference by these reagents. The use of lipemia removal methods other than centrifugation-based methods may involve the introduction of a reagent that might interfere with the measurement of certain biochemical parameters.

Our results do not support the use of LipoClear, in agreement with those of Saracevic et al [ 12 ], who concluded that LipoClear is not suitable for lipemia removal from samples to measure concentrations of glucose, sodium ion, potassium ion, chloride ion, phosphorus, magnesium, creatine kinase MB, alkaline phosphatase, gamma-glutamyl transferase, protein, albumin, and C-reactive protein.

In addition, 1,1,2-trichlorotrifluoroethane has been found to interfere with the measurement of certain biochemical parameters, including gamma-glutamyl transferase, C-reactive protein, and creatine kinase MB [ 11 , 12 ]. In our study, 1,1,2-trichlorotrifluoroethane did not interfere with glutamyl transferase measurements. LipoClear and 1,1,2-trichlorotrifluoroethane are not suitable because they interfere with the measurement of certain biochemical parameters.

Methods Seven hospital laboratories in Spain participated in this study. Results When ultracentrifugation and high-speed centrifugation were compared, no parameter had a difference that exceeded the LCSI. Study design and biochemical analyses This was a prospective and transversal study involving seven hospital laboratories in Spain: Gran Canaria Dr.

Statistical analysis Grubbs test was used to detect aberrant results. The authors declare that they have no conflicts of interest. Kroll M. Evaluating interference caused by lipaemia. Clin Chem ;50; Serum indices : managing assay interference. Ann Clin Biochem ;53; Incidence and amount of turbidity, hemolysis, and icterus in serum from outpatients.

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Clin Chem Lab Med ;45; CMV IgG. Colon cancer. Complete blood count CBC. Complete Polysomnography Sleep Study. Complete profile. Copper plasma.

Coronary Heart Disease. Creatinine and eGFR. Creatinine Urine. Cystine Crystals. D D-dimers. D2 Dermatophagoides farinae. Density routine urine test. Direct Bilirubin. Direct Coombs. E ECG. Egg White. Egg Yolk. Eggs and Parasites 1st, 2nd or 3rd specimen. Electroencephalogram EEG. Erythrocytes routine urine test. Factor VIII. Fat Bodies routine urine test. Fecal Calprotectin. Fecal Immunochemical Test.

Femoral-Patellar Syndrome. Flu influenza. Folic acid and erythrocyte folates. Free Kappa and Lambda. Free T3. Free T4. Free Testosterone. G G6PD Quantitative. Gamma globulins. Glucose routine urine test. Granular Casts. Grass Mix 1. Growth Hormone. Gynecological Cytology.

H Hallux valgus. HDL Cholesterol. Heel spur. Helicobacter pylori Breath Test. Helicobacter pylori Serology. Hemochromatosis Genotype. Hemoglobin Electrophoresis. Hepatitis B. Hepatitis C IgG. Herniated disc. Hiatal hernia. HLA B Hot zone. Howell-Jolly Body. HS Troponin T. Hyaline Casts. I Icterus. IGF 1. Iliotibial Band Friction Syndrome. Immunity and herd immunity.

Inhibin B. Insomnia acute or chronic. Ionized Calcium. Iron profile. Irritable bowel syndrome. K Karyotype. Ketones routine urine test. Kidney Cells. Kidney Disease nephropathy. L Lactiferous ducts. Lateral epicondylitis. LDL Cholesterol. Leflunomide Metabolite. Leukocytes esterase. Leukocytes routine urine test. Liver profile. Lumbar Facet Syndrome. Lumbar osteoarthritis. Lumbar sprain. Lying Aldosterone. Lying Renin. Lyme Disease. Lymphogranuloma venereum.

M Macadamia Nuts. Maintenance of Wakefulness Test. Mean Corpuscular Hemoglobin. Mean Corpuscular Volume. Mean Platelet Volume. Medial epicondylitis. Meniscal lesion. The data used to support the findings of this study are included in the article. Previously reported data were used to support this study and are available at DOI: Neda Soleimani and Fateme Asadian conducted the study including patient recruitment, data collection, and analysis. Both authors approved the final manuscript.

This is an open access article distributed under the Creative Commons Attribution License , which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Article of the Year Award: Outstanding research contributions of , as selected by our Chief Editors. Read the winning articles. Journal overview. Special Issues. Academic Editor: Boryana M. Received 14 Nov Accepted 21 Jan Published 12 Feb Abstract Introduction.

Materials and Methods 2. Samples This study was conducted in the clinical laboratory of Shahid Motahari Clinic an outpatient department of Shiraz University of Medical Sciences from August to September of Statistical Analysis The mean and standard deviation were calculated for each parameter in all groups of lipemia and also for all four methods.

Figure 1. Percentage of bias for 21 biochemistry analytes in different levels of lipemia. Table 1. Chemical methods, wavelength, and analytical range used for analysis.

Table 2. Comparison of the results of native serum, serum blank, and diluted serum with ultracentrifuged serum. Table 3. Table 4. References D. Dimeski and B. Lima-Oliveira, W. Volanski, G. Lippi, G. Picheth, and G. View at: Google Scholar R.

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