What is dcmu

The actinic light was turned on for 1 s at the zero time point. Measurements were done for each of the treatments on three different days with the same results; a representative set is shown. As with all RNA blots shown in this report, the same blots are shown hybridized with an rnpB probe as a loading control.

Inhibitors were added in the last 5 min of LL adaptation to allow time for the cells to absorb the inhibitors and then cells were exposed to light conditions for 20 min before harvesting for RNA. Zero time point samples for RNA were then taken and rifampin was added.

Incubation was continued under HL for an additional five min, at which time zero-time points samples for RNA were taken, and cultures were maintained in HL. Subsequent time-point samples for all samples for RNA were taken at the indicated times following rifampicin addition.

RNA extracted from each of the samples was subjected to Northern hybridization analysis with an hliA -specific probe. Data was obtained by densitometry of the autoradiograms. The average values of two independent repetitions of the experiments were subjected to exponential regression analysis and are plotted, and the range between repetitions is indicated. Top A Northern-blot hybridization analysis of hliA transcript levels is shown.

LL-adapted cells were kept in LL or transferred to the dark or low-intensity red light RL for 10 min. Adamska, I. Edited by Baker, N. Alfonso, M. Plant Physiol. PCC A redox control mechanism. Allen, J. FEBS Lett. Anderson, J. Berg, S. Bhaya, D. Natl Acad. USA 96 : — FEMS Microbiol. Clarke, A. Cremer, K. Danon, A. Science : — Demmig-Adams, B. Plant Mol. Dolganov, N. USA 92 : — El Bissati, K. Escoubas, J.

Fankhauser, C. Cell Dev. Fujita, Y. In The Molecular Biology of Cyanobacteria. Edited by Bryant, D. Kluwer Academic Publishers, Dordrecht. Funk, C. Biochemistry 38 : — Garcia-Dominguez, M. Glatz, A. Golden, S. Green, B. Trends Biochem. Havaux, M. Acta : 21 — He, Q. Hihara, Y. With an accout for my. DCMU 3- 3,4-dichlorophenyl -1,1-dimethylurea is a herbicide that inhibits photosynthesis. It was released by Bayer in under the name of Diuron. DCMU is a very specific and sensitive inhibitor of photosynthesis.

It blocks the plastoquinone binding site of photosystem II, disallowing the electron flow from where it is generated, in photosystem II, to plastoquinone. This interrupts the photosynthetic electron transport chain in photosynthesis and thus blocks the ability of the plant to turn light energy into chemical energy ATP and reductant potential. DCMU only blocks electron flow from photosystem II, it has no effect on photosystem I or other reactions in photosynthesis, such as light absorption or carbon fixation in the Calvin cycle.

Besides its use a herbicide, DCMU is often used to research energy flow in photosynthesis. Categories: Herbicides Toxicology. Death of the plant may result from starvation due to obstructed photosynthesis, from the action of harmful radicals generated as a result of continued light absorption by a blocked photosynthetic electron transport system, or from a combination of both these alternatives.

Inhibition of the photoheterotrophic growth of plant cell cultures by photosynthetic herbicides 4 supports the toxic radical hypothesis 5 , but these results were obtained using concentrations of herbicides much higher than necessary to inhibit photosynthetic electron transport in vivo and may thus have revealed non-specific effects of the herbicides.

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